Human lncRNA Splicing-targeting CRISPR Library

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Human lncRNA Splicing-targeting CRISPR Library

Human lncRNA Splicing-targeting CRISPR Library

Catalog# LIBR-H023-P002 LIBR-H023-P100 LIBR-H023-P200 LIBR-H023-P500

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Instruction

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The CRISPR knockout library targets 10,996 human lncRNA with a total of 126,773 knockout plasmid vectors, of which 11 different gRNA vectors are designed for each lncRNA, in addition to 500 negative control vectors targeting intergenic sequences as well as 350 positive control vectors targeting essential genes. The library uses pLenti-sgRNA(lib)-puro as the backbone, which is a two-plasmid system that expresses only gRNA, while the Cas9 gene is on a separate vector that needs to be used in conjunction.
Product Name
Human lncRNA Splicing-targeting CRISPR Library
Species
Human
Library Type
Knockout Library
Plasmid System
Dual-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
10996
gRNA Number
126773
Non-targeting gRNA Number
850
Selection Marker
Puro
Backbone Map
pLenti-sgRNA(lib)-puro vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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